Throughout lead identification and lead optimization phases of drug development, initial hit compounds are tested in dose-response assays (EC50, IC50, ADME Tox assays). Those assays are fundamental experiments in pharmacology and toxicology, aimed at understanding the relationship between the dose of a substance and the extent of the response it provokes in a biological system (i.e. efficacy and toxicity).

Dose-response curves allow for the comparison between different substances and thus their clinical and therapeutic values.

 

 

 

 

 

The BIOSPOT® workstation simplifies dose-response curve generation with the PIPEJET® direct aspiration and dispensing capabilities at highest precision.

 

 

 

Precise non-contact dispensing, adjustable from 7 - 15 nL per drop

Sustainable use of consumables with the optional pipe cleaning, which eliminates the need to manually replace pipes and allows them to be reused

Trust the precision of every drop through automate volume calibration and QC

Full flexibility on deck: up to 6 plates freely addressable with no need for plate handling or inverting

 

 

 

 

Compound addition using the BIOSPOT® workstation

Typically, compound stocks (usually 10 mM in DMSO) are cherry-picked from various storage formats, prediluted, and subsequently dispensed into assays plates. The concentration curves generally range from 7 to 11 data points, with each point increasing by half-logarithmic increments.

The automated setup of concentration curves with the BIOSPOT® workstation allows for precise nanoliter dispensing without the use of multiple tips. Dispensing in nanoliter volumes opens up the possibility of achieving lowest possible compound concentrations while keeping the DMSO level to a minimum.